Fig. 1: Cryo-EM characterization of the endogenous 80S-RAC complexes.
From: Structural remodeling of ribosome associated Hsp40-Hsp70 chaperones during co-translational folding
a Overview of the cryo-EM map of the 80S-RAC-eEF2-eIF5A-Stm1 complex, a representative 3D class of the endogenous 80S-RAC complexes. The density map is from Map 1.7 in Supplementary Fig. 2. For purpose of illustration, EM density of RAC is from another map with the RAC region improved by local classification (Map 1.2). The 40S and 60S subunits are colored light cyan and gray, respectively. Ribosome binding factors Stm1, eEF2, eIF5A and RAC are shown in yellow, blue, red and orange, respectively. b Subunit-interface view of the endogenous 80S-RAC-eIF5A-eEF2-Stm1 complex. For clarification, only the 60S subunit is shown. The functional centers of the ribosome are highlighted in the right panel. DC, Decoding center; PTC, Peptidyl transferase center; PTE, Peptide tunnel exit. The mRNA entry channel on the 40S subunit is occupied by a helix from Stm1.
