Fig. 4: Structure of the RNC-RAC complex with nearly full density of RAC.
From: Structural remodeling of ribosome associated Hsp40-Hsp70 chaperones during co-translational folding
a Overall structure of the State C2 from the RNC-RAC dataset. The density map is from Map 3.5 in Supplementary Fig. 9. The ribosome is colored gray, and Zuo1 ND, JD, ZHD and Ssz1 are shown in green, blue, orange and pink, respectively. b Segmented density map of RAC, superimposed with the models of Zuo1 and Ssz1. The subdomains of both proteins are labelled, and the flexible sequence of Zuo1 (50–70) is indicated in a gray dash-line. The density map is from Map 3.6 in Supplementary Fig. 9. c Magnified view of the intermolecular interface between Zuo1 JD and Ssz1 SBD. Zuo1 ND partially blocks the HPD motif (highlighted in cyan) of Zuo1 JD. The polar interface between the surface of Zuo1 JD helices and Ssz1 SBD is highlighted by circled “+” and “-” symbols. d Ribosomal contacts of Ssz1 at the PTE. Ssz1 interacts with uL24 and H7-loop, a component of the 25S rRNA, through its subdomain NBD-IIB. Ssz1 and uL24 are shown in pink and light yellow, respectively. H7-loop, with or without Ssz1 interactions, is colored light blue and apricot, respectively. Selected residues of Ssz1, uL24 and H7-loop are shown in stick models and labelled.
