Fig. 6: IL2 mutein is efficacious in acute GvHD and EAE mouse models.

Acute GvHD model: A Body weights of F1 animals following adoptive transfer of B6 cells. Animals (n = 6 for all groups except n = 2 for NA group) were dosed on days −3, 0, 3, 7 and 10 as indicated by arrows. Asterisks denote significance (two-way ANOVA, two-sided, p-value * < 0.05, ** < 0.01) compared to PBS group and data are presented as mean ± s.d. Frequency of donor B B cells and C CD8 T cells. D Frequency of IFNγ producing CD8 T cells. E correlation of Treg frequency and CD8 T cell IFNγ expression. EAE model: animals were dosed on days −3, 0, 3 and 6. F Disease score in murine EAE model (n = 12), statistics shown for HSA-IL2m against both PBS and HSA groups (Kruskal–Wallis). G Frequency of Tregs (FOXP3+) among CD4 T cells in spleen and inguinal lymph nodes (n = 8). H Frequency of MOG-specific (Tetramer+, Supplementary Fig. 15) Th1 (T-bet+) cells in spleen and inguinal lymph nodes (n = 8). I Frequency of IFNγ expression in splenic antigen experienced CD4 T cells (n = 8). Asterisks denote significance (two-way ANOVA, two-sided, p-value * < 0.05, ** < 0.01, *** < 0.001). Box plots center is the median, bounds of box are 1st and 3rd quartile. Upper whisker is the largest value that is no greater than 1.5× the interquartile range plus the third quartile. Lower whisker is the smallest value that is no smaller than the first quartile minus 1.5× the interquartile range. Source data are provided as a Source Data file.