Fig. 7: In vivo bioorthogonal NIR imaging for 4T1 tumor-bearing mice.

a Timeline of RGD-TCO, CyP7T injection, and NIR imaging. Mice were pre-injected with TCO-RGD (10 nmol) or saline, followed by injection of CyP7T (5 nmol) 12 h later. b NIR imaging was performed at various timepoints (0, 0.5, 1, 2, 4, 8, 12, 24, 48 h) post-injection of CyP7T. c Fluorescence signal intensity in tumor tissue and d Tumor-to-background ratio (TBR) over time, n = 3. TBR was determined by (tumor ROI—background ROI)/background ROI. e Fluorescence intensity analysis of excised organs harvested from mice 24 h post-injection of CyP7T. Data are presented as mean ± SD. (n = 3 biologically independent mice per group).