Fig. 2: The radula transcriptome and expression of key genes controlling limpet tooth formation.
From: Biomimetic generation of the strongest known biomaterial found in limpet tooth
a Principal component analysis (PCA) demonstrating the relatedness of transcript expression between limpet muscle, the FZ and radula Stages I to IV. b Heat map showing the abundance (Z-score normalised log FPKM for RNA seq) for all genes with differential expression across the limpet muscle, the FZ and radula transcriptomes. c Venn diagram showing overlap of gene expression in the FZ, the radula and muscle. d–e Overlap between the genes upregulated, and downregulated respectively, in the radula compared to muscle, which was used as a common control. f–g Log FPKM values for RNA seq for genes involved in chitin and iron metabolism, respectively. CHS Chitin synthase, CHIT Chitinase, CNNM2 Cyclin and CBS domain divalent metal cation transport mediator 2, FRRS1 Ferric chelate reductase 1, HEPHL Hephaestin-like protein 1, HIP Heavy metal-binding protein HIP, STEA3 metalloreductase six-transmembrane epithelial antigen of prostate 3, TRFM Melanotransferrin.
