Fig. 6: Thiolysis analysis of the soluble extracts and the insoluble fractions of 7 DAP siliques of ans-4 mutant in the presence of excess Cys.
From: A role for ascorbate conjugates of (+)-catechin in proanthocyanidin polymerization
a UHPLC-QqQ analysis of AsA-C with MRM transition of m/z (463.1 → 175.0), and Cys-C and Cys-EC with MRM transition of m/z (408.1 → 125.0) in the soluble extracts before and after thiolysis and the insoluble fractions after thiolysis in the presence excess Cys. sd: standard. Note that the ion abundances are not directly comparable among different types of samples, as different dilution factors were applied to each sample to prevent the over-saturation of ion abundance in the QqQ detector. b The mDP of soluble AsA-[C]n in the ans-4 mutant and soluble PA in WT. Data are shown as the mean ± SD (for n = 3 biologically independent samples). c The concentration of AsA-C from different fractions and free Cys-C in ans-4 mutant sample. FW: fresh weight. Data are shown as the mean ± SD (for n = 3 biologically independent samples; ***P < 0.001, two-tailed unpaired Student’s t tests). Source data of Fig. 6b and c are provided as a Source Data file.
