Fig. 9: Levels of AsA-[C]n, Cys-C, and (+)-catechin-type extension unit involved in PA polymerization in grape berry skins and seeds at different E-L developmental stages before harvest.
From: A role for ascorbate conjugates of (+)-catechin in proanthocyanidin polymerization
a Levels of AsA-[C]n, Cys-C and (+)-catechin-type extension unit involved in PA polymerization in grape berry skins. b Levels of AsA-[C]n, Cys-C and (+)-catechin-type extension unit involved in PA polymerization in grape seeds. MRM transitions of m/z (463.1 → 175.0), m/z (751.0 → 287.0), m/z (1039.0 → 863.0) and m/z (408.1 → 125.0) were used for the detection of AsA-C, AsA-C-C, AsA-C-C-C and Cys-C respectively on UHPLC-QqQ. (+)-Catechin-type extension unit in the soluble PAs after Sephadex LH-20 resin purification and insoluble PAs were represented by Cys-C after thiolysis with the excess of Cys. Levels of the compounds are represented by the peak area on UHPLC-QqQ. Data are shown as the mean ± SD (for n = 3 biologically independent samples; the different letters above the bars represent statistically significant differences (at P < 0.05) determined by one-way ANOVA with Tukey’s multiple comparisons test). Source data of Fig. 9a and b are provided as a Source Data file.
