Fig. 2: RBM17 is required to support primitive leukemic cell functions.

a Western blot validation of RBM17 knockdown (KD) in transduced GFP⁺ HL60 cells. n = 3 independent experiments. b, c Flow cytometric evaluation of myeloid differentiation (P(shscramble vs shRBM17#1)=0.0108, P(shscramble vs shRBM17#2)=0.0011)) (b) and apoptosis (P(shscramble vs shRBM17#1)=0.0111, P(shscramble vs shRBM17#2)=0.0039) (c) following RBM17 knockdown in primary AML cells. n = 3, mean ± SD, two-tailed Student’s t test. d–h Colony formation capacity assessed in 5 AML samples following RBM17 KD. n = 3, mean ± SD, two-tailed Student’s t test. i Representative flow cytometry plots showing the GFP⁺ cell percentage in the human cell population post-transplant for AML#001 (top) and total human grafts (CD45⁺CD33⁺ cell percentage) post-transplant for AML#006 (bottom). j Quantification of AML engraftment after 9 weeks (#001) in whole bone marrow. Shown is the ratio of the GFP⁺ cell percentage in the human cell population post-transplant to the initial pre-transplant GFP⁺ cell percentage. Data are presented as mean ± SD, n = 5, two-tailed Student’s t test, P(shscramble vs shRBM17#1)=0.0415, P(shscramble vs shRBM17#2)=0.0173. k AML engraftment (#006) after 12 weeks in bone marrow. Shown is the percentage of human (CD45⁺) myeloid cells (CD33⁺) found in bone marrow. n = 5, mean ± SD, two-tailed Student’s t test, P(shscramble vs shRBM17#1)=0.0026, P(shscramble vs shRBM17#2)=0.0002. l–n Representative histogram (l) and quantification (m, n) of flow cytometric immunophenotyping of myeloid differentiation in post-transplant grafts from j, k. n = 5, mean ± SD, two-tailed Student’s t test. o CFU output from transduced Lin^- CD34⁺ cord blood (CB). n = 5, mean ± SD, two-tailed Student’s t test. p Relative engraftment of CD34⁺CD38^- enriched normal CB HSC with and without RBM17 knockdown. Shown is the ratio of the GFP⁺ cell percentage in the human cell population post-transplant to the initial pre-transplant GFP⁺ cell percentage. n = 6, mean ± SD, two-tailed Student’s t test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Source data are provided as a Source Data file.