Fig. 4: Efficient transfer of vaccine-induced antibodies to cord blood.

A The dot plots show the Spike-specific IgG1 titer or FcR3a binding for maternal plasma (M) and cord blood (C). Lines connect maternal-cord dyads and the color represents vaccine type, Ad26.COV2.S (red), mRNA-1273 (yellow) or BNT162b2 (blue). Significance was determined by Wilcoxon signed-rank test (2-sided) followed by posthoc Benjamini–Hochberg correction adjustment, *p < 0.05, **p < 0.01, ***p < 0.001. Exact p-value for FcR3a: mRNA-1273 p = 0.0000052, BNT162b2 p = 0.042. B The dot plots show the Spike-specific antibody-dependent cellular phagocytosis (ADCP), antibody-dependent neutrophil phagocytosis (ADNP), antibody-dependent complement deposition (ADCD), and antibody-dependent NK cell degranulation, as measured by % CD107a + NK cells, for maternal plasma (M) and cord blood (C). Lines connect maternal-cord dyads and the color represents vaccine type, Ad26.COV2.S (red), mRNA-1273 (yellow) or BNT162b2 (blue). Significance was determined by Wilcoxon signed-rank test (2-sided) followed by posthoc Benjamini–Hochberg correction adjustment, *p < 0.05, **p < 0.01, ***p < 0.001. Exact p-values stated in the following order for all subpanels: mRNA-1273 M-C, then BNT162b2 M-C, unless otherwise noted. ADCP: p = 0.00011, p = 0.0066. ADNP: p = 0.00007, p = 0.0099. ADCD: BNT162b2 p = 0.011. CD107a+: p = 0.00021, p = 0.047. C–E A multilevel PLSDA (mPLSDA) was built for Ad26.COV2.S (C), mRNA-1273 (D) and BNT162b2 (E) using sample type, maternal blood (M, light purple) or cord blood (CB, dark purple) as the outcome variable. Features were selected using LASSO prior to building the models. The dot plots (top) show the scores plots for the mPLSDA. Each dot represents a sample, with the color representing the sample type. The ellipses represent the 95% confidence interval for the sample type. The bar plots show the LV1 for the mPLSDA built in each respective subfigure. Source data are presented in Source Data File 1.