Fig. 3: ATRX enrichment at regulatory elements varies with the activity of the locus and ATRX binding sites conserved across cell lines show conserved chromatin accessibility states.

a Distribution of the ATRX binding sites relative to genes in erythroblasts. b Distribution of the ATRX binding sites depending on their chromatin accessibility as assessed by ATAC-seq in erythroblasts. c Motif analysis of ATRX binding sites in erythroblasts (p-values HOMER findMotifsGenome.pl)⁵⁸. d Representative image of the α-globin locus active in erythroblasts and silenced in LCLs. The signals represent an average of the independent replicates (in LCLS, n = 3 for ATRX ChIP-seq, H3K4me1, H3K4me3, H3K27me3 and H3.3 ChIP-seq, n = 2 for H3K27ac ChIP-seq and n = 4 for ATAC-seq. In erythroblasts, n = 3 for ATRX-ChIP-seq, n = 1 for H3K4me1, H3K4me3, H3.3 and H3K27me3 and n = 4 for ATAC-seq). e Venn diagram showing the cell type-specific and conserved ATRX binding sites in LCLs and erythroblasts. f Distribution of the conserved ATRX binding sites based on their position in relation to genes. g Chromatin accessibility status in LCLs and erythroblasts at the conserved ATRX binding sites. h Distribution of the conserved ATRX binding sites based on the presence or absence of PQS. i Genome-wide ATRX enrichment across genes in LCLs depending on the gene expression status in LCLs and erythroblasts. The signals represent an average of the independent replicates (n = 3). j Genome-wide ATRX enrichment across genes depending on the level of gene expression in LCLs. The signals represent an average of the independent replicates (n = 3).