Fig. 3: In vivo studies showing that C. auris BJCA001 induces a less potent innate immune response than C. albicans SC5314.

a Quantitative fungal burden of spleen, kidney, and brain of C57BL/6 mice (n = 5 mice/group/time point) intravenously infected with either 1 × 10⁶ (L) or 2 × 10⁷ (H) of C. auris yeast cells at day 2 and 5 post-inoculations. For comparison, a parallel experiment was conducted using C. albicans (5 × 10⁴ CFU; n = 4 mice/group/time point). b, c Shown are neutrophil populations in spleen (b) and kidney (c) cell suspensions of mice that were infected without (PBS, negative control) or with live C. auris (n = 5) or C. albicans (n = 4) for 2 and 5 days. The number of neutrophils was determined by flow cytometry. C. albicans was injected into mice at an inoculum of 5 × 10⁴ CFU and C. auris was injected at an inoculum of 1 × 10⁶ CFU(L) or 2 × 10⁷ CFU (H). d, e Shown are the major immune cell populations in spleen (d) and kidney (e) cell suspensions of mice treated as in b. The number of each immune population was determined by flow cytometry. f Serum cytokine and chemokine levels, as determined by ELISA, in mice treated as in a. Data are expressed as mean ± SD and are representative of three independent experiments. ns, no significance; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; by two-way ANOVA with Tukey’s test (b–e) or one-way ANOVA with Dunnett’s test (f). Source data are provided as a Source Data file.