Fig. 1: The mATG8-conjugation system is not required for PINK1–Parkin-mediated mitochondria clearance. | Nature Communications

Fig. 1: The mATG8-conjugation system is not required for PINK1–Parkin-mediated mitochondria clearance.

From: A degradative to secretory autophagy switch mediates mitochondria clearance in the absence of the mATG8-conjugation machinery

Fig. 1: The mATG8-conjugation system is not required for PINK1–Parkin-mediated mitochondria clearance.The alternative text for this image may have been generated using AI.

a WT or ATG7-KO HeLa stably expressing mCherry-Parkin were treated with antimycin A and oligomycin (A/O, 1 µM each) for the indicated duration. Mitochondria content was assessed by immunoblotting of proteins from different mitochondria compartments. b Quantification of mitochondrial protein changes from (a). Mean of n = 4 independent replicates ± SEM is shown. c WT, ATG5-KO, or ATG3-KO HeLa stably expressing mCherry-Parkin were treated with A/O for the indicated duration. d Quantification of mitochondrial protein changes from (c). Mean of n = 4 independent replicates ± SEM is shown. e Representative immunofluorescence images of WT, ATG7-KO and ATG5-KO cells stably expressing YFP-Parkin treated with A/O for 24 h and stained for mtDNA (red) and DAPI (blue). Scale bar = 10 µm. f Quantification of mtDNA volume from (e). Box plots indicate median (middle line), 25th, 75th percentile (box) and outlier limits (whiskers) with individual measurement data points overlaid. P values in (b, d) were calculated by two-way ANOVA followed by Dunnett’s multiple comparisons test against the 0 h timepoint. *P  <  0.05, **P  <  0.01, ***P  <  0.001, and n.s. denotes not significant. See source data for exact P values.

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