Fig. 2: FD ampoules and DNA damage of FD somatic cell nuclei.
From: Healthy cloned offspring derived from freeze-dried somatic cells
a Ampoules of FD somatic cells. b Damaged cell membrane after rehydration. Left: bright field; middle: Hoechst staining; right: propidium iodide (PI) staining. n = 4 biologically independent ampoules. c, d Comet DNA breakage assays of FD somatic cell nuclei. FT: Freeze-thaw. FD-tre: Freeze-dry with trehalose. FD-epi: Freeze-dry with epigallocatechin. n = 3 biologically independent experiment. a vs. b: P < 0.05 (one-way ANOVA and Tukey-multiple comparison-test). See Supplementary Table 1 for more details. Data are presented as mean value, minima and maxima.
