Table 2 Production of cloned mice from freeze-dried somatic cell via ntES cells.
From: Healthy cloned offspring derived from freeze-dried somatic cells
Donor cell type | Sex | Treated with | Mouse strain | No. of oocytes used | No. of activated oocytes | No. of PN formed oocytes | No. of embryos developed to 2-cell stage (/PN) | No. of embryo transfer | No. of offspring (/ET)[/PN] |
|---|---|---|---|---|---|---|---|---|---|
Cumulus | F | Treha | BDF1 | 3146 | 2695 (85.7) | 2356 (74.9) | 1400 (59.4) | 1327 | 3 (0.2) [0.1] |
Epigallo | BDF1 | 2369 | 2116 (89.3) | 1875 (79.1) | 1016 (54.2) | 1016 | 16 (1.6) [0.9] | ||
Fibroblast | M | Epigallo | BDF1 | 1868 | 1647 (88.2) | 1460 (78.2) | 867 (59.4) | 864 | 47a (5.4) [3.2] |
Epigallo | BCF1 | 1200 | 1012 (84.3) | 884 (73.7) | 520 (58.8) | 520 | 9 (1.7) [1.0] |
