Fig. 2: Gphn expresses a myriad of GPHN protein isoforms.
From: Complex regulation of Gephyrin splicing is a determinant of inhibitory postsynaptic diversity
A Filtering pipeline used in this study to generate GPHN theoretical proteome, (i) Translation Initiation Start (TIS) identification, (ii) retained ORFs including at least 300 nucleotides, (iii) ORFs initiated with a TIS confirmed by biological evidences, (iv) removal of duplicated ORFs, (v) withdrawal of ORFs including PTC. B Workflow to isolate GPHN protein isoforms present at GABA-A and Glycine inhibitory synapses and MS processing to analyze them. GPHN was isolated from whole-brain lysate via affinity purification using a neurotransmitter receptor peptide (FSIVGRYRRRC; KD (GPHN) = 140 nM). Nano-LC MS/MS identified 2428 unique GPHN peptides, in which 71 corresponded to 148 novel GPHN protein isoforms. Source data are provided as a Source Data file.
