Fig. 4: GPHN isoforms have distinct synaptic properties.
From: Complex regulation of Gephyrin splicing is a determinant of inhibitory postsynaptic diversity
Eleven Scarlet-tagged GPHN isoforms were analyzed in hippocampal primary neuronal culture. Exogenous GPHN isoforms were selected from the pool of most expressed Gphn transcripts in cortex and cerebellum (Fig. 1D). A Representative images of global localization of GPHN isoforms (red) in neuronal cells. B Confocal analysis of proximal dendrites in neuronal cells expressing Scarlet or Scarlet-tagged GPHN isoforms (red), presynaptic side was stained with anti-GAD-65 (green). C Density of GPHN clusters along the dendrite. D Size of the GPHN clusters. E Percentage of GPHN clusters associated with a GAD-65 punctum. F Density of GAD-65 puncta along the dendrite. For experiments performed in C–F, at least 12 cells were analyzed in n = 6 independent experiments for C, E, and F, and n = 5 independent experiments for D. Bar graphs show means ± SEM. ****P < 0,0001, ***P < 0,0010, **P < 0,01, *P < 0,05. One-way analysis of variance (ANOVA) for C–E. *P < 0,05. Kruskal–Wallis test for (F). Scale bar: 25 µm (A) and 15 µm (B). Source data is provided as a Source Data file.
