Fig. 5: Increase of GPHN isoform levels modulates the number of inhibitory synapses in mouse cerebellum.
From: Complex regulation of Gephyrin splicing is a determinant of inhibitory postsynaptic diversity
Analysis of inhibitory synapses connected to Purkinje cells in mouse cerebellar slices after transduction of lentivirus expressing Scarlet-tagged GPHN isoforms. A Schematic displaying the experimental procedure. B Cerebellar cortex slices in which inhibitory synapses are stained at pre- and postsynaptic sides using anti-GAD65 antibody (green) and Scarlet fluorescence (red), respectively (n = 5 mice for each isoform). C Quantification of GAD-65 density detected in panel B (n = 5 independent experiments). Box boundaries in C are the 25th and 75th percentiles, the horizontal line across the box is the median, and the whiskers indicate the minimum and maximum values. ***P < 0,0010, *P = 0,0179 and *P = 0,0128 for GPHN8 and GPHN49, respectively. Two-sided Student's t-test. Scale bar: 15 µm. Source data is provided as a Source Data file.
