Fig. 5: Cellular experiments with JMneu EGFR show reduced phosphorylation.

a Expression of phosphorylated EGFR and total EGFR at 0, 5, 15, and 30 min of 100 ng/mL EGF (top) and 300 ng/mL of epigen (bottom) stimulation in CHO cells transfected with WT EGFR and JMneu EGFR. Actin was used as a loading control. The blots reported are representative of seven independent biological replicates. Black line delineates boundary between gels. b Relative expression of phosphorylated tyrosine (pTyr) was determined by western blot quantification. The values were normalized to pTyr before EGF or epigen stimulation (time 0) of the wild-type EGFR transfected cells. Data are presented as mean values +/−SEM from seven independent biological replicates (circles); average, SEM reported in Supplementary Table 6. Two-tailed, nonparametric, paired t-test was performed to obtain the P-values (exact P-values mentioned in Supplementary Table 7). No adjustments were made for multiple comparisons. Source data are provided as a Source data file. c Schematic of proposed effect of JMneu EGFR on phosphorylation in CHO cells. The positive region in the JMD is shown in blue in WT EGFR and in dotted blue in JMneu EGFR. The negative lipids and region in the NCTT are shown in red. The circles in the CTT indicate tyrosine phosphorylation (P) sites.