Fig. 4: Increased IL-10 levels in the lungs mediate bacterial expansion in lung.
From: Host lung microbiota promotes malaria-associated acute respiratory distress syndrome
a Total mRNA (Kruskal–Wallis) and b protein levels (Kruskal–Wallis test followed by Wilcoxon post hoc analysis) of IL-10 in the lungs of C57BL/6J mice 5 days p.i. with Pb K173 (n = 6; N = 2) or Pb ANKA (n = 6; N = 2) compared to NI controls (n = 6; N = 2). c Survival (upper panel, Log-rank Mantel-Cox) and parasitemia (lower panel, mean ± s.e.m.; linear regression) following Pb K173 infection of IgG (n = 10; N = 2) or αIL-10R neutralizing antibody (n = 10; N = 2) treated C57BL/6J mice. d Survival (upper panel, Log-rank Mantel-Cox) and parasitemia (lower panel, mean ± s.e.m.; linear regression) following Pb ANKA infection of IgG (n = 10; N = 2) or αIL-10R neutralizing antibody (n = 10; N = 2) treated C57BL/6J mice. e Total CFUs (Kruskal–Wallis test followed by Wilcoxon post hoc analysis) in the lungs of C57BL/6J mice 5 days p.i. with Pb K173 (n = 6; N = 2) treated with αIL-10R neutralizing antibody and compared to IgG treated (n = 6; N = 2) and NI (n = 6; N = 2) controls (n.d. = not detected). f Parasite sequestration (Kruskal–Wallis test followed by Wilcoxon post hoc analysis) in the lungs of C57BL/6J mice 5 days p.i. with Pb K173 (n = 6; N = 2) treated with αIL-10R neutralizing antibody and compared to IgG treated (n = 6; N = 2) and NI (n = 6; N = 2) controls. g IL-10 protein levels (Kruskal–Wallis test followed by Wilcoxon post hoc analysis) in the lungs of C57BL/6J mice 5 days p.i. with Pb K173 treated with linezolid starting 3 days p.i. (n = 4; N = 1) compared to NI (n = 4; N = 1) and non-treated, Pb K173 (n = 4; N = 1) infected controls. h Parasite sequestration (Kruskal–Wallis test followed by Wilcoxon post hoc analysis) in the lungs of C57BL/6J mice 5 days p.i. with Pb K173 treated with linezolid starting 3 days p.i. (n = 4; N = 1) compared to NI (n = 4; N = 1) and non-treated, Pb K173 (n = 4; N = 1) infected controls. a, b, e–h Data are represented as floating bar plots (minimum to maximum) with line at the middle representing median. Source data are provided as a Source Data file.
