Fig. 1: Construction of a yeast pan-genome neo-chromosome.

a Schematic representation of the circular PGNC construct (PGNC^circ). b Iterative assembly of the PGNC via homologous recombination and using alternating selectable markers. c Confirmation of the PGNC assembly using PCR reactions that span each inter-chunk junction. Identical PCR results were obtained across at least two repetitions and during sub-assembly d Production of three linear PGNC variants using the telomerator (PGNC^lin1, PGNC^lin2 and PGNC^lin3). Yellow boxes define the position of the centromeric-plasmid backbone. Red boxes denote positions of synthetic telomeric repeat sequences added by the telomerator process. e Microplate growth kinetics of the four PGNC variants (PGNC^circ, PGNC^lin1, PGNC^lin2 and PGNC^lin3) compared to the BY4742 (WT) strain. Data are presented as mean values +/− SD, based upon values obtained from three independent biological replicates. Source data are provided as a Source Data file.