Fig. 2: Mitotic stability of the PGNC in the absence of selection.
From: Construction of a synthetic Saccharomyces cerevisiae pan-genome neo-chromosome
a Populations were established for each four PGNC variants (PGNCcirc, PGNClin1, PGNClin2 and PGNClin3) and serially passaged eight times for ~50 generations of total growth. b Differential plating of 96 colonies for phenotypic assessment of loss of the PGNC element. c Results of passaging experiments (96 colonies per replicate) for each of the PGNC strains after both 25 and 50 generations. d Results of passaging experiments (96 colonies per replicate) for each of the PGNC strains containing additional ARS sequences after both 25 and 50 generations. All graphs are presented as mean values +/− SD, based upon values obtained from three independent biological replicate passaging experiments. Source data are provided as a Source Data file.
