Fig. 1: Combined high glucose and high fat up-regulates islet miR-21 expression.

a Pancreatic islets were isolated from 8-week-old male C57BL/6 mice and treated with or without high glucose and high fat as indicated in the figure. After incubating for 24 h at 37 °C, total RNA was extracted and relative miR-21 expression level was determined by quantitative RT-PCR. Data are presented as means ± SD for n = 3 biologically independent samples. Statistical significance was analyzed using one-way ANOVA with Bonferroni correction. P values are indicated in the figures. ns not significant. LG low glucose (5.5 mM), HG high glucose (25 mM), NF no fat, HF high fat (0.4 mM sodium palmitate). b Pancreatic islets were isolated from 6-week-old male diabetic db/db mice and control BKS mice (n = 3). Total RNA was extracted and relative miR-21 expression level was determined by quantitative RT-PCR. Data are presented as means ± SD for n = 3 biologically independent samples. c Pancreatic islets were isolated from 20-week-old male ob/ob mice and control C57BL/6 mice (n = 3). Relative miR-21 expression level was determined as in (b). Data are presented as means ± SD for n = 3 biologically independent samples. For (b) and (c), statistical significance was analyzed using two-sided unpaired t test and P values are indicated in the figure. ns not significant. All data shown are representative of three independent experiments. Source data are provided as a Source Data file.