Fig. 1: High-resolution immunophenotypic and transcriptome mapping of steady-state granulocytic and monocytic differentiation hierarchies. | Nature Communications

Fig. 1: High-resolution immunophenotypic and transcriptome mapping of steady-state granulocytic and monocytic differentiation hierarchies.

From: Transcription factor-driven coordination of cell cycle exit and lineage-specification in vivo during granulocytic differentiation

Fig. 1

a–c Flow cytometry-based gating strategy for sorting of murine bone marrow (BM) populations representing early stages of myeloid (i.e., granulocytic-monocytic) differentiation (a) and late stages of granulocytic (b) and monocytic differentiation (c). d PCA plot of murine BM populations clustered based on the similarity of gene expression profiles. Replicates of BM populations are encircled. a Early myeloid (i.e., granulocytic-monocytic) differentiation hierarchy: LIN-SCA + KIT + hematopoietic stem and early progenitor (LSK), pre-granulocyte-monocyte progenitors (preGM), and granulocyte-monocyte progenitors (GMP). b Late granulocytic differentiation hierarchy: Granulocyte progenitors (GP), promyelocytes (PM), early and late myelocytes (MY1, MY2), metamyelocytes (MM), band cells (BC), and fully mature granulocytes (GR). c Late monocytic differentiation hierarchy: Monocyte progenitors (MP), early and late promonocytes (PMO1, PMO2), and early and late monocytes (MO1 and MO2). Source data are provided as a Source Data file.

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