Fig. 6: CEBPA and CEBPE binding dynamics to cis-regulatory elements during early and late granulocytic differentiation. | Nature Communications

Fig. 6: CEBPA and CEBPE binding dynamics to cis-regulatory elements during early and late granulocytic differentiation.

From: Transcription factor-driven coordination of cell cycle exit and lineage-specification in vivo during granulocytic differentiation

Fig. 6

a Regions bound by CEBPA during early granulocytic differentiation in LSKs, preGMs, and GMPs demonstrate significant overlap and the most abundant binding of CEBPA in GMPs. b Regions bound by CEBPE in MY1s + MY2s, MMs, BCs, and GRs demonstrate significant overlap of CEBPE binding and the most abundant binding of CEBPE in MY1s + MY2s and MMs. c Regions bound by CEBPA in GMPs and by CEBPE in MY1s + MY2s demonstrate a high overlap of CEBPA and CEBPE binding during early late granulocytic differentiation. d Heatmap showing the levels of CEBPA, CEBPE, H3K4me1, and H3K27ac at promoters and enhancers (>1000 bp from TSS) for indicated BM populations, along with their target gene clusters. Shown alongside the CEBPE binding motif score for each region are the E2F1 and MYC binding affinities for the same regions in embryonic stem cells (ESC). Also shown are the H3K4me1 (priming marker) and H3K27ac (activation marker) levels at enhancers for indicated BM populations. e, f CEBPA binding (e) and H3K27ac modification (f) levels at regions bound by CEBPA and/or CEBPE (i.e., CEBPA & E, CEBPA, CEBPE) for the indicated populations. Source data are provided as a Source Data file.

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