Fig. 2: Deep U-Net based femto-SRS imaging with recovered chemical resolution.

a Single raw femto-SRS image (middle) was converted by U-Net into the spectrally resolved images at 2845 cm−1 and 2930 cm−1 (right), compared with the ground truth pico-SRS images (left). b Intensity profiles (dashed lines in (a)) of the predicted and ground-truth data, showing chemical contrast in the cell nucleus regions (shadows, and arrows in (a)). c, d Femto-SRS imaging of live cells and gastric tissues, yielding almost identical results to pico-SRS. Green: lipids; blue: protein; red: collagen fibers. Scale bars: 50 µm. Source data are provided in the Source data file.