Fig. 4: Pat1 enables evasion of recognition by autophagy.
From: A patatin-like phospholipase mediates Rickettsia parkeri escape from host membranes
Images of autophagy receptors a NDP52 (left; magenta in merge) and b p62 (right; magenta in merge) in WT and pat1::Tn (green in merge) infected HMECs. Asterisk denotes colocalization between bacterium and p62 or NDP52. Scale bars in (a, b) 5 µm. c Quantification of (a) and (b), percentage of bacteria staining for NDP52 or p62 at 1 hpi (>1000 bacteria counted per strain for each marker), MOI 3. Data are mean ± SEM; **p = 0.0060, *p = 0.0354 relative to WT (unpaired t-test (two-tailed)). d Images of LC3 (magenta) in HMECs infected with WT and pat1::Tn bacteria (green) at 1 hpi. Asterisk denotes colocalization between bacterium and LC3. Scale bars 5 µm. e Quantification of percent bacteria staining for LC3 at 1 hpi (images in (d)) and 2 hpi (images not shown), (>1500 bacteria counted per strain for each time point), MOI 3. Data are mean ± SEM; *p = 0.0244 (1 hpi), p = 0.0124 (2 hpi) relative to WT (unpaired t-test (two-tailed)). f Percentage colocalization of bacteria with polyUb, NDP52, and p62 in HMECs infected with WT, pat1::Tn mutant, or co-infected with WT and pat1::Tn mutant (>700 bacteria counted for each strain/marker), MOI 5. For co-infections, quantification is for pat1::Tn bacteria only. Data are mean ± SEM; ***p = 0.0001, **p = 0.0030 (p62), p = 0.0016 (NDP52, pat1::Tn), p = 0.0023 (NDP52, co-infection) relative to WT (one-way ANOVA with Dunnett’s post hoc test). All data represent n = 3 independent experiments. Source data are provided as a Source Data file. .
