Fig. 3: The SR1/R2 and \(R{S}_{{{{\rm{R}}}}1/{{{\rm{R}}}}2}^{{{{\rm{Mutant}}}}/{{{\rm{WT}}}}}\) values of the WT and mutants. | Nature Communications

Fig. 3: The SR1/R2 and \(R{S}_{{{{\rm{R}}}}1/{{{\rm{R}}}}2}^{{{{\rm{Mutant}}}}/{{{\rm{WT}}}}}\) values of the WT and mutants.

From: Engineering substrate specificity of HAD phosphatases and multienzyme systems development for the thermodynamic-driven manufacturing sugars

Fig. 3: The SR1/R2 and $$R{S}_{{{{\rm{R}}}}1/{{{\rm{R}}}}2}^{{{{\rm{Mutant}}}}/{{{\rm{WT}}}}}$$ R S R 1 / R 2 Mutant / WT values of the WT and mutants.The alternative text for this image may have been generated using AI.

a–c represented the SM6P/G6P and SG6P/M6P values, SM6P/F6P and SF6P/M6P values, and SG6P/F6P and SF6P/G6P of WT and different mutants, respectively. d The SM6P/R2 values of WT and mutant MM3 for M6P over substrates 2DG6P, R5P and E4P. e The SF6P/R2 values of WT and mutant MF2 to F6P over other three substrates. f The SG6P/R2 values of WT and mutant MG7 to G6P over other three substrates. g–i represented the substrate preference change compared with WT. For example, \({{RS}}_{{{\mbox{F}}}6{{\mbox{P/M}}}6{{\mbox{P}}}}^{{{\mbox{MF}}}2{{\mbox{/WT}}}}\) value was calculated by the ratio of SF6P/M6P of MF2 to that of the WT. 2DG6P:2-deoxy-d-glucose-6-phosphate; R5P: d-ribose-5-phosphate; E4P: d-erythrose-4-phosphate. Source data for Fig. 3 are provided as a Source Data file.

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