Fig. 5: Biosynthesis of fructose from starch and sucrose by constructing multienzyme systems. | Nature Communications

Fig. 5: Biosynthesis of fructose from starch and sucrose by constructing multienzyme systems.

From: Engineering substrate specificity of HAD phosphatases and multienzyme systems development for the thermodynamic-driven manufacturing sugars

Fig. 5: Biosynthesis of fructose from starch and sucrose by constructing multienzyme systems.The alternative text for this image may have been generated using AI.

a Thermodynamically-driven manufacturing fructose from starch. The standard Gibbs free energy change of each and overall reaction was presented. The ΔG° value was freely available on the website. http://equilibrator.weizmann.ac.il. (Pi)n: hexametaphosphate, (Amyl)n: maltodextrin. b Thermodynamically-driven manufacturing fructose from sucrose. c Biosynthesis of fructose under 100 g/L of maltodextrin. The reaction medium containing maltodextrin (100 g/L), PBS buffer (30 mM, pH 6.5), MgCl2 (5 mM), TmGP (10 U/mL), TkPGM (5 U/mL), TtPGI (10 U/mL), and MF2 (10 U/mL) was carried out at 55°C for 24 h. After reaction for 12 h, 5.0 U/mL of Tl4GT, 5.0 U/mL of TfPPGK, and 50 mM of hexametaphosphate were added to the reaction solution. d The effect of enzyme amount of BaSP on PF values. e Biosynthesis of fructose under 100 g/L of sucrose. The reaction medium comprising of 100 g/L of sucrose, 20 mM of PBS buffer (pH 6.5), 5 mM of MgCl2, 2.5 U/mL of BaSP, 5 U/mL of TkPGM, 10 U/mL of TtPGI, and 10 U/mL of MF2 was carried out at 55 °C for 12 h. Data are presented as mean values +/−SD (n = 3 independent experiments). Source data for c–e are provided as a Source Data file.

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