Fig. 3: Single-cell transcriptome analysis of porcine ECs in pig tissues.

a Heatmap of gene expression in the selected PECAM+/EPCAM−/PTPRC−/COL1A1−/PDGFRB− ECs from 19 tissues. b t-SNE visualization of 21 EC clusters, which are colored according to EC cluster numbers. c Bar graph shows numbers and percentage of ECs in tissues. ECs were colored according to clusters. d EC subtypes in adipose tissues. Cells are color-coded according to the subtypes of ECs. e EC subtypes trajectory analysis in adipose tissues (adipose-S and adipose-V) using monocle 2 and cells on the tree are colored by EC subtypes. f EndMT EC trajectory analysis using monocle 2 and cells on the tree are colored by EndMT states. g Pseudotime trajectory analysis of marker genes in different states of EndMT cells. h Representative pseudotime trajectory of marker genes (TGFB2, SNAI1, PECAM1, VWF, ZEB2, ACTA2, TAGLN, NOTCH3, FABP4) in different states of EndMT ECs. i Representative Immunofluorescence staining images of VWF and TAGLN in adipose tissues. Arrow (red) indicates ECs expressing both VWF and TAGLN. Arrow (green) indicates ECs only expressing VWF (n = 3). j Representative Immunofluorescence staining images of PECAM1 and ACTA2 in adipose tissue. Arrow (red) indicates ECs expressing both PECAM1 and ACTA2. Arrow (green) indicates ECs only expressing PECAM1 (n = 3). k IHC of FABP4 in ECs and adipose tissues. Arrows (green) indicate ECs. Arrows (red) indicate adipocytes. Control was stained with an antibody against a gene not expressed in adipose tissues (n = 3). l An integrated model of EndMT process. Source data are provided as a Source Data file. Schematic diagrams in I were created with BioRender.com.