Fig. 4: Validation of EndMT ECs in cultured ECs from pig lung and aorta by scRNA-seq. | Nature Communications

Fig. 4: Validation of EndMT ECs in cultured ECs from pig lung and aorta by scRNA-seq.

From: Endothelial cell heterogeneity and microglia regulons revealed by a pig cell landscape at single-cell level

Fig. 4: Validation of EndMT ECs in cultured ECs from pig lung and aorta by scRNA-seq.The alternative text for this image may have been generated using AI.

a The flowchart of ECs isolation and culture from pig lung and aorta. b tSNE visualization of cell-type annotations from cultured lung ECs. c Dot-plot of selected canonical marker genes for cell-type annotations from cultured lung ECs. d tSNE visualization of cell-type annotations from cultured aorta ECs e Dot-plot of selected canonical marker genes for cell-type annotations from cultured aorta ECs. f Cell cycle analysis of annotated cell types in cultured lung and aorta ECs. g Expression of ACTA2 and CD31 in cultured PAEC treated with TGFb2 for five days (n = 3, two-sided t-test). Values are presented as mean±SD. h Expression of ACTA2 and CD31 in cultured HUVEC treated with TGFb2 for 5 days (n = 3, two-sided t test). Values are presented as mean ± SD. Source data are provided as a Source Data file.

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