Fig. 3: Assessment of cerebral pathology in heme pathway KO parasite-infected mice.

a Evans blue extravasation in the brain of mice infected with PbWT and heme pathway PbKO parasites. b Quantification of Evans blue in the brain samples of mice infected with PbWT (n = 6) and heme pathway PbKO (n = 9) parasites. (mean ± SD; ***P < 0.001, unpaired t-test; two-sided). c H&E staining of the brain sections prepared from PbWT and PbKO parasite-infected mice. Black arrows—intracerebral and petechial hemorrhages, blue arrows—thrombosed blood vessels and brown arrows—gross demyelination. Images were captured using 10x objective. Scale bar = 50 μm. n = 3–5 independent experiments. d IgG extravasation in the brain sections of PbWT and PbKO parasite-infected mice. Black arrows—areas showing IgG immunoreactivity. Images were captured using 10x objective. Scale bar = 50 μm. n = 3 independent experiments. e H&E staining of the brain sections indicating (black arrows) occluded vasculatures containing luminal and abluminal leukocytes, and parasite-derived Hz. Images were captured using 60x objective. Scale bar = 10 μm. n = 3 independent experiments. f Immunofluorescence analysis of parasite accumulation in the brain sections of PbWT and PbKO parasite-infected mice. n = 3 independent experiments. g Immunofluorescence analysis of CD3⁺ cells in the blood vessels of PbWT and PbKO parasite-infected mice. n = 2 independent experiments. h Immunofluorescence analysis of β-APP staining in the brain sections of PbWT and PbKO parasite-infected mice. n = 2 independent experiments. f–h Images were captured using 20x objective. Scale bar = 20 μm. Source data are provided as a Source Data file.