Fig. 2: Contribution of direct neurons to threshold crossing varied between session 1 and the LP session.

a Neural activity of the direct neurons at the time of success was categorized into eight strategies. The eight strategies accounted for 94.7% of all successful trials across the four mice (Figs. S8–11). Trial strategies were defined as follows: a single DP or DN neuron contributed to 70% or more of the control signal generated at the time of success, or either the summed activity of the 3 DP or the 3 DN neurons coordinated to contribute to 70% or more of the control signal at the time of success. In a minority of cases the strategy did not fall into one of these categories, such trials are referred to as unclassified. b The normalized real-time fluorescence at the time of success was significantly higher (K–S test) in the LP session (n = 150 trials) compared to session 1 (n = 216 trials) in DP neurons #2 and #3 (DP neuron #2, p = 2.26E−16; DP neuron #3, p = 1.92E−05) and lower in DP neuron #1 (p = 5.31E, Supplementary Table 2) in mouse #1. See Supplementary Table 2 for the change in mean values in all four mice. c Activity of the 6 direct neurons during successful trials, aligned to the time of success, in session 1 and the LP session for mouse #1. Some trials were shorter than 2 s (gray background). Note, DP neuron #2 was the dominant contributor to threshold crossings in the LP session. Source data are provided as a Source Data file.