Fig. 4: Conformational state of the C-terminal lobe of human Ago2 upon binding of guide and target RNA. | Nature Communications

Fig. 4: Conformational state of the C-terminal lobe of human Ago2 upon binding of guide and target RNA.

From: Single-molecule FRET uncovers hidden conformations and dynamics of human Argonaute 2

Fig. 4

Doubly labeled apo hAgo2N/Mid (A) and binary hAgo2N/Mid-guide RNA complexes (B) were immobilized via an hAgo2-directed antibody, whereas ternary hAgo2N/Mid-guide-target complexes (C) were immobilized via the target RNAlong-Biotin. All FRET efficiency histograms show data of at least three independent measurements. All data were fitted using a Gaussian equation. Source data provided as a Source Data file.

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