Fig. 8: Reporters generated from Exd-independent ScrT1 > UbxT3 peaks. | Nature Communications

Fig. 8: Reporters generated from Exd-independent ScrT1 > UbxT3 peaks.

From: Transcription factor paralogs orchestrate alternative gene regulatory networks by context-dependent cooperation with multiple cofactors

Fig. 8

a Table summarizing the leg disc expression patterns driven by selected Exd-independent ScrT1 > UbxT3 CRMs. b, c Examples of Dll bound Exd-independent ScrT1 > UbxT3 peaks from dsx (dsx-1; b) and dpy (dpy-1; c) that drive T1 ≠ T3 expression patterns in leg discs. On the left are genome browser tracks for the ScrT1, UbxT3, ScrT1/+, Scr(YPWM*)T1/+, DllT1 and DllT3 ChIP-seq signals, as well as ATACT1 and ATACT3 signals. Hox ChIP-seq peaks within the CRMs are numbered. The vertical gray bars denote the Hox ChIP peak center region that alters reporter expression when deleted. The dsx-1 and dpy-1 probes in Fig. 7 are also derived from the deleted regions. Panels on the right show T1 and T3 leg discs immunostained for reporter gene expression. The T1- and T3-specific expression patterns are indicated by arrows. Note that dsx-1 drives expression only in male T1 leg discs, as expected for a dsx leg CRM. Scale bar: 100 μm.

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