Fig. 3: Identification of multiple CD8+ TIL populations by single cell RNA sequencing.
From: CD8+T cell responsiveness to anti-PD-1 is epigenetically regulated by Suv39h1 in melanomas
A Scheme of the overall study design. Single–cell RNA sequencing was applied to CD8+ TILs isolated from littermate WT and Suv39h1-KO mice receiving B16F10-OVA melanoma cells followed by PBS or anti-PD-1 Ab. B UMAP plot with clusters differentiated by colour demonstrating eight distinct CD8+ TILs clusters based on gene expression differences for 21646 passing quality control cells. Each dot corresponds to one single cell. C Feature plots and violin plots showing distribution of signature scores indicated by cluster. D Violin plots showing expression distribution of representative genes for each cluster. E Heatmap showing Z scores for the average expression of selected genes within the eight clusters. See also Supplementary Fig. 4. Source data are provided as a Source Data file.
