Fig. 5: Suv39h1-deficiency enhances anti-PD-1-induced chromatin opening at TCR-, cytolytic-, and IFNa-responsive sites.

A Number of chromatin accessible regions in CD8+ TILs of littermate WT and Suv39h1-KO mice treated or not with anti-PD-1. B ATAC-seq regions in littermate WT and Suv39h1-KO modulated by anti-PD-1 treatment. Venn diagram showing anti-PD-1 treatment modulated peaks in littermate WT, Suv39h1-KO, or both (overlap) (middle). Chromatin accessibility heat map grouped by modulated peaks by anti-PD-1 treatment in CD8⁺ TILs of littermate WT, Suv39h1-KO, or both (bottom). C Violin plots showing distribution of anti-PD-1 induced ATAC-seq signals in CD8+ TILs littermate WT and Suv39h1-KO CD8+ TILs. Central line represents median, box represents quartiles, whiskers represent minimal to maximal data value. D Motif enrichment analysis showing top transcription factor motifs enriched in peaks differentially accessible in CD8+ TILs of the described group comparisons. E Venn diagram showing number of genes adjacent to anti-PD-1 induced ATAC-seq peaks in littermate WT and Suv39h1-KO CD8+ TILs (top). Selected signatures enriched in anti-PD-1 induced adjacent peaks open in littermate WT and Suv39h1-KO CD8+ TILs determined through hypergeometric test alternative greater (middle). Representative ATAC-seq tracks showing accessibility peaks across the loci of Egr1, Mx1, Cx3cr1 and Gzmb for littermate WT and Suv39h1-KO treated or not with anti-PD-1 CD8⁺ TILs (bottom). F Chromatin accessibility heat map grouped by unique differentially accessibility regions in CD8⁺ TILs of littermate WT+ anti-PD-1 and Suv39h1-KO+ anti-PD-1 (top). Violin plots showing distribution of unique anti-PD-1 signals in CD8+ TILs of littermate WT+ anti-PD-1 and Suv39h1-KO+ anti-PD-1. Central line represent median, box represent quartiles, whiskers represent minimal to maximal data value. All ATAC-seq data are representative of two biologically independent pooled samples. Source data are provided as a Source Data file.