Fig. 1: Experimental design for CO identification in Arabidopsis inbred lines.
From: The megabase-scale crossover landscape is largely independent of sequence divergence
M2 plants were derived from the selfing of independent EMS-treated seeds. Pairs of M2s were crossed to produce F1*s, which are then heterozygous for a set of unique mutations defining two phases, indicated by coloured ticks. Two F1*s were then reciprocally crossed to generate F1 populations. The DNA of leaves of F1* and F1 plants were sequenced using Illumina. The colour-coded ticks indicate EMS-induced mutation markers. The red triangles represent COs, detected by phase switching. This design allows the detection of COs that occurred in the female and male meiocytes of the F1* plants. The sequencer figure was created with BioRender.com.
