Fig. 4: Machine-learning strategy infers transcription factors mediating the transcriptional effects of ISO and lipolysis.
From: Lipolysis regulates major transcriptional programs in brown adipocytes
Mouse in vitro differentiated brown adipocytes were pre-treated with 10 µM Atglistatin (ATGL inhibitor) and 20 µM CAY10499 (HSL inhibitor) for 1 h and subsequently with 100 nM isoproterenol (ISO) or 20 µM SR-3420 for 3 h before harvesting for ChIP and ATAC. a Heatmap of H3K27ac levels in ATAC regions (ISO vs. vehicle and SR-3420 vs. vehicle: Log2FC ≥ 0.5 or Log2FC ≤−0.5) of mouse-brown adipocytes stimulated ISO or SR-3420 for 3 h before harvest. The results are 2 independent biological experiments. b K-means clustering of motif activity (calculated using IMAGE) of 127 transcription factor motifs, mRNA levels, and reported function of the 23 lipolysis-dependent transcription factor motifs in mouse-brown adipocytes.
