Fig. 5: SunCatcher enables identification and quantification of early spontaneous metastasis.
From: Clonal barcoding with qPCR detection enables live cell functional analyses for cancer research
a Calibration curves were generated for indicated tissues by serially diluting known amounts of barcoded tumor cell gDNA into a fixed amount of normal tissue gDNA. From left to right: lung, long bones (from femur and tibia), mandible. b 2.5 × 105 barcoded Met1 tumor cells were injected bilaterally into the mammary fat pads (n = 5 animals) and tumors were allowed to grow for 21 days, at which point tissues were harvested and metastasis burden was calculated. Dashed lines indicate the background signals from each indicated tissue type. Tissues with signal above the background were considered positive for metastasis and estimated tumor cell number per 0.1 mg tissue was calculated based on the calibration curve for that specific tissue. c Barcode composition analysis on tissues with positive metastasis signal. Bars represent percent of total barcode signal (100%) within each sample. Also shown are mouse identities, total primary tumor burden for each animal, and estimated numbers of metastases per tissue; N.D., not detected. Source data are provided as a Source Data file.
