Fig. 3: Impaired surface trafficking of a subset of SLITRK2 variants.

a Schematic diagrams depicting the SLITRK2 variants analyzed in the current study. b Representative immunoblots from HEK293T cells transfected with the indicated WT or mutant forms of SLITRK2. Samples containing equal amounts of protein were resolved by SDS-PAGE and immunoblotted using anti-HA or GFP antibodies; β-actin was used as a loading control. Molecular mass markers are labeled in kilodaltons. The experiments were independently repeated three times. c Surface expression analysis of HEK293T cells expressing the indicated WT or mutant forms of SLITRK2. Transfected cells were immunostained with mouse anti-HA antibodies (cyan) and detected with FITC-conjugated anti-mouse secondary antibodies under non-permeabilized conditions, followed by permeabilization of cells. Cells were then stained first with rabbit anti-HA antibodies (magenta) and then with Cy3-conjugated anti-rabbit secondary antibodies. Scale bar, 10 μm (applies to all images). d Quantification of the proportion of cells exhibiting surface expression. All data are shown as means ± SEMs (‘n’ denotes the number of images from three independent experiments; WT, n = 10; L74S, n = 7; E210K, n = 9; P311A, n = 7; T312A, n = 6; P374R, n = 7; R426C, n = 8; E461*, n = 8; R484Q, n = 6; V511M, n = 6; E555D, n = 6; R792C, n = 7; S323N, n = 13; V589I, n = 13; V201I, n = 16; WT, n = 7; S9I, n = 7; and G15E, n = 7; **p < 0.01, ***p < 0.001; ANOVA with a non-parametric Kruskal–Wallis test). See Source data for raw data values and Supplementary Table 4 for statistical details.