Fig. 4: Impaired dendritic targeting of a subset of SLITRK2 variants in cultured hippocampal neurons. | Nature Communications

Fig. 4: Impaired dendritic targeting of a subset of SLITRK2 variants in cultured hippocampal neurons.

From: SLITRK2 variants associated with neurodevelopmental disorders impair excitatory synaptic function and cognition in mice

Fig. 4: Impaired dendritic targeting of a subset of SLITRK2 variants in cultured hippocampal neurons.The alternative text for this image may have been generated using AI.

a Representative images of hippocampal neurons transfected with the indicated WT or mutant forms of SLITRK2 at DIV10. The transfected neurons at DIV14 were double-immunostained for antibodies against the somatodendritic marker, MAP2 (cyan), and HA (magenta). Scale bar, 20 μm (applies to all images). b, c Somatic (b) or dendritic (c) targeting of WT or the indicated mutant forms of SLITRK2 in hippocampal neurons was quantified by measuring average intensity of HA immunofluorescence in primary dendrites. The average intensities of SLITRK2 WT and variants in the soma region of transfected neurons were also quantified. All data are shown as means ± SEMs (‘n’ denotes the number of neurons from three independent experiments; WT, n = 12; L74S, n = 10; E210K, n = 10; P311A, n = 10; T312A, n = 10; P374R, n = 11; R426C, n = 10; E461*, n = 11; R484Q, n = 10; V511M, n = 10; E555D, n = 11; R792C, n = 10; S323N, n = 10; V589I, n = 10; V201I, n = 10; **p < 0.01, ***p < 0.001, ****p < 0.0001; ANOVA with a non-parametric Kruskal–Wallis test). See Source data for raw data values and Supplementary Table 4 for statistical details.

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