Fig. 7: Alterations of full-length TrkB protein levels in cultured neurons by a subset of SLITRK2 variants. | Nature Communications

Fig. 7: Alterations of full-length TrkB protein levels in cultured neurons by a subset of SLITRK2 variants.

From: SLITRK2 variants associated with neurodevelopmental disorders impair excitatory synaptic function and cognition in mice

Fig. 7

Cultured cortical neurons were infected with lentiviruses expressing WT or the indicated mutant forms of SLITRK2. Levels of TrkB were examined by semi-quantitative immunoblotting using antibodies against TrkB or phospho-TrkB. FL full-length, T truncated. b Quantification of protein levels from (a). Summary data showing plots from five independent experiments. Data are mean ± SEMs (*p < 0.05; ANOVA with a non-parametric Kruskal–Wallis test). c Hippocampal CA1 lysates from Slitrk2-floxed mice infected with AAVs expressing ΔCre or Cre were examined by semi-quantitative immunoblotting using antibodies against TrkB or phospho-TrkB. FL full-length, T truncated. d Quantification of protein levels from (c). Data are means ± SEMs (n = 5 mouse samples; *p < 0.05; two-tailed unpaired t-test). See Source data for raw data values and Supplementary Table 4 for statistical details.

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