Fig. 5: Benazepril inhibits Mtb Alr in vitro.
From: Low-cost anti-mycobacterial drug discovery using engineered E. coli
His-tagged Mtb Alr was purified and incubated with the indicated drug concentrations. Alr activity, VF, was assayed using a coupled reaction yielding NADH. a Inhibition curves for benazepril and DCS. Data is presented as the man and CI95 of three independent assays. b In vitro kinetics of Alr varying both inhibitor and substrate. At least three individual measurements are plotted for each pair of concentrations. Lines indicate best-fit Michaelis–Menten curves for each tested benazepril concentration. c Best-fit values of Michaelis–Menten parameters as a function of benazepril concentration. Data is presented as the mean and CI95 for best-fit parameter values, as estimated using Student’s t-distribution. Source data are provided as a Source Data file.
