Fig. 1: Bs GS forms tetradecamers in the presence of TnrA and GlnR. | Nature Communications

Fig. 1: Bs GS forms tetradecamers in the presence of TnrA and GlnR.

From: Molecular dissection of the glutamine synthetase-GlnR nitrogen regulatory circuitry in Gram-positive bacteria

Fig. 1: Bs GS forms tetradecamers in the presence of TnrA and GlnR.The alternative text for this image may have been generated using AI.

A 2D class averages of negative stain EM images of apo Bs Gs, Bs FBI-GS-TnrA, and Bs FBI-GS-GlnR complexes. Right shows a close-up of the top views revealing that apo Bs GS is a dodecamer, while Bs FBI-GS-TnrA and Bs FBI-GS-GlnR are tetradecamers. B Fluorescence polarization (FP) based assays analyzing Bs FBI-GS, Sa FBI-GS, Pp FBI-GS, and Lm FBI-GS binding to their corresponding fluoresceinated GlnR C-tails with Kds of 4.2 ± 0.3 μM, 7.2 ± 0.3 μM, 18.7 ± 3.3 μM, and 27.2 ± 3.4 μM, respectively. The curves are representative curves from three technical repeats. The error bars represent SD. Data were presented as mean values ± SD. The source data are provided in the Source Data file.

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