Fig. 8: GlnR-DNA contacts. | Nature Communications

Fig. 8: GlnR-DNA contacts.

From: Molecular dissection of the glutamine synthetase-GlnR nitrogen regulatory circuitry in Gram-positive bacteria

Fig. 8: GlnR-DNA contacts.The alternative text for this image may have been generated using AI.

A FP based assays analyzing binding to the 21 bp operator by FL and truncated Sa GlnR, which resulted in Kds of 9.9 ± 0.9 nM and 75 ± 8.0 nM, respectively. The curves are representative curves from three technical repeats. The error bars represent SD. Data were presented as mean values ± SD. Source data are provided in the Source Data file. B Schematic representation of Sa, Bs, and Lm GlnR contacts to DNA. Residues that contact the DNA from Sa, Bs, and Lm GlnR are indicated in salmon, cyan, and slate, respectively. C Close-up view of the DNA contacts from each GlnR subunit to a half-site (contacts by the other subunit to the other half-site are identical).

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