Fig. 4: PSC-derived Ihh orchestrates bone growth.
From: Periosteal stem cells control growth plate stem cells during postnatal skeletal growth
a Scatter plot of the gene expression ratio. b Ihh expression in periosteal osteogenic progenitors and CTSK– bone marrow mesenchymal stem cells (n = 4 biologically independent samples). P values were calculated using ANOVA with Tukey’s multiple-comparison test. Data are presented as the mean ± S.D. c Ihh expression in various periosteal cell types (n = 5 and 3 mice per group). P values were calculated using one-sided Student’s t-test. Data are presented as the mean ± S.D. d Images of littermates. Ihhflox/+, Ihhflox/flox, Ihhflox/+ Ctsk-Cre mice were phenotypically identical, being grouped together and used as controls in (d–h). e Body weight of littermates (n = 11 and 3 mice per group). P values were calculated using one-sided Student’s t-test. Data are presented as the mean ± S.D. f Representative femur images of more than three littermates. Scale bars, 2 mm. g Representative femur images analyzed in h. Scale bars, 1 mm. The white arrow heads indicate bone width in the control mice. h μCT analysis parameters of the femur in littermates (n = 11 and 3 mice per group). P values were calculated using one-sided Student’s t-test. Data are presented as the mean ± S.D. i Representative skull images of more than three female littermates. Scale bars, 7 mm. j Representative haematoxylin and eosin staining pictures of the tibiae growth plate in more than three littermates. Scale bars, 100 μm. R: resting zone, P: proliferating zone, H: hypertrophic zone. k Frequency of RZSCs in 3-week-old littermates (n = 4 mice per group). P = 0.0051. P value was calculated using one-sided Student’s t-test. l RZSC number in 3-week-old littermates (n = 4 mice per group). P value was calculated using one-sided Student’s t-test. Data are presented as the mean ± S.D. m Mean Fluorescence Intensity of CD73 in RZSCs (n = 4 mice per group). P value was calculated using one-sided Student’s t-test. Data are presented as the mean ± S.D. n Ki67+ cell frequency in RZSCs in littermates (n = 4 mice per group). P value was calculated using one-sided Student’s t-test. Data are presented as the mean ± S.D. o The expression levels of Ptch and Gli1 in RZSCs (n = 4 mice per group). P values were calculated using one-sided Student’s t-test. Data are presented as the mean ± S.D. Source data are provided as a Source Data file.
