Fig. 2: Primary DMSXL astrocytes display abnormal population dynamics. | Nature Communications

Fig. 2: Primary DMSXL astrocytes display abnormal population dynamics.

From: Myotonic dystrophy RNA toxicity alters morphology, adhesion and migration of mouse and human astrocytes

Fig. 2: Primary DMSXL astrocytes display abnormal population dynamics.The alternative text for this image may have been generated using AI.

a xCELLigence cell index of primary astrocytes and primary neurons over 72 h. Data are mean ± SEM, n = 6 independent cultures per group, each one established from a different animal (p < 0.0001, Two-way repeated measures ANOVA). b Percentage of DMSXL and WT astrocytes in G1, S, and G2/M phases of the cell cycle by FACS analysis of DNA content (PI levels) and DNA synthesis (BrdU incorporation). Data are mean ± SEM, n = 3 independent cultures per genotype. c FACS analysis of DMSXL and WT astrocyte cell death under control conditions (DMSO) and in the presence of Staurosporine (Staur). Percentage of apoptotic cells labeled with annexin V (AnxV+), and necrotic cells labeled with propidium iodide (PI+). Data are mean ± SEM, n = 6 independent cultures per genotype (p = 0.0043, WT; p = 0.0240, DMSXL; Two-way ANOVA and Sidak post hoc test for multiple comparisons). Source data are provided as a Source Data file. *p < 0.05; **p < 0.01; ***p < 0.001.

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