Fig. 2: Auristatin activity in murine models. | Nature Communications

Fig. 2: Auristatin activity in murine models.

From: Monomethyl auristatin antibody and peptide drug conjugates for trimodal cancer chemo-radio-immunotherapy

Fig. 2

a Nude or C57BL/6 mice injected with saline or Cy5-labeled ADC on day 0. Blood Cy5 plotted as mean ± SD, n = 3. b Mice with orthotopic CAL27 tongue xenografts injected with 2.5 nmoles of CDX3379-MMAE (n = 4) or saline (n = 2) and tumors harvested 48 h. Tumor drug concentration scatter plot. c Mice implanted orthotopically with CAL27 parental (WT) or resistant (PIK3CA H1047R) cells on day 0 injected with 2.5 nmoles drug on day 7. Tumor volume plotted as mean ± SEM, n = 6. Statistical significances calculated using two-way ANOVA with Tukey’s multiple comparisons test. Tumor burden in resected tongues. d Mice implanted with MOC2 cells on day 0 injected with 2.5 nmoles drug on day 7. Tumor volumes plotted as mean ± SEM, n = 4. Statistical significance calculated using two-way ANOVA with Tukey’s multiple comparisons test. Tumors analyzed on day 11 by CyTOF using t-distributed stochastic neighboring embedding (tSNE). Concatenated image delineating immune cell discrimination. Scatter plot with mean ± SEM of untreated and CDX3379-MMAE, n = 4; CDX3379, n = 3. Statistical significances calculated using one-way ANOVA with Tukey’s multiple comparisons test. e For MOC1 tumors, mice injected on day 0 (tumor volume ∼40 mm3) with 2.5 nmoles CDX3379-MMAE (n = 5) or untreated (n = 4). For B16, mice implanted with tumor cells on day 0 and injected on day 3 with 5 nmoles CDX3379-MMAE (n = 10), cetux-MMAE (n = 5) or untreated (n = 9). Mouse survival plotted and statistical significance of CDX3379-MMAE to control or cetux-MMAE calculated using Log-rank (Mantel–Cox) test with single comparisons. f B16 cell surface binding of Cy5-labeled ADCs measured by flow cytometry and plotted as percent of maximum. g Cy5-labeled ACPP injected into mouse with orthotopic 4MOSC1 tumor, tongue resected, imaged for Cy5 (white signal, left image), sectioned (right image), nuclei DAPI stained (blue) and Cy5 captured (red). h Mice implanted with orthotopic 4MOSC1 cells on day 0 injected with 10 nmoles drug on days 5 and 7 (n = 8) or untreated (n = 9). Tumor volumes plotted as mean ± SEM. Statistical significance calculated using two-way ANOVA with Tukey’s multiple comparisons test. Source data provided in Source Data file.

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