Fig. 3: Thy1+ cells replenish the adult mouse dorsal epidermis over time.
From: Thy1 marks a distinct population of slow-cycling stem cells in the mouse epidermis
a Genetic strategy used to induce multicolor Confetti expression in Thy1+ cells. b Schematic of induction and harvesting regimen of the dorsal skin. c Image of Confetti-labeled cells in the basal layer after 1 day post induction [n = 5 mice]. d Frequency of clones comprised of more than one Thy1+ labeled cell at 3 days post induction [n = 90 clones from 4 pooled mice]. Source data are provided as a Source Data file. e Confocal image of sectioned epidermis at day 30 post-induction showing Confetti expression throughout the epidermal layers [n = 5 mice]. Dashed white line demarcates epidermis/dermis boundary. f Z-stack images of induced dorsal epidermis after 30 days showing formation of labeled cornified units [n = 5 mice]. g 3D rendered confocal image of Thy1-CreERT2;R26EYFP dorsal skin at 30 days post induction co-stained with Thy1 (yellow arrows) [n = 5 mice]. h Z-stack sections of dorsal skin interfollicular epidermis (IFE) at 30 days post induction showing basal attachment. Dotted white lines marks polygonal projection of a cornified cell [n = 5 mice]. i–m Maximum projection confocal images of Thy1+ basal cell-derived clones at (i) 7 days, (j) 16 days, (k) 30 days, (l) 60 days, and (m) 365 days post induction [n = 4 mice per time point]. Scale bars: 100 μm (k–m), 50 μm (e, f, h–j), 20 μm (c, g).
