Fig. 9: Comparison of experimentally validated FDR of MSn methods. | Nature Communications

Fig. 9: Comparison of experimentally validated FDR of MSn methods.

From: Mimicked synthetic ribosomal protein complex for benchmarking crosslinking mass spectrometry workflows

Fig. 9: Comparison of experimentally validated FDR of MSn methods.The alt text for this image may have been generated using AI.

DSSO cross-linked main library were measured on an Eclipse Tribrid Mass Spectrometer using a stepped HCD MS2, MS3 and CID MS2- ETD MS2 acquisition strategy. Data was analyzed using XlinkX at 1% FDR. Red bars indicate false crosslinks whereas green bars represent true unique crosslinks. Error bars indicate standard deviations of average values, experimentally validated FDR is shown as callout, n = 3. Source data are provided as a Source Data file.

Back to article page